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Image Search Results
Journal: Cell
Article Title: Clonal Evolution of Autoreactive Germinal Centers
doi: 10.1016/j.cell.2017.07.026
Figure Lengend Snippet: (A) Representative immunofluorescence analysis of IgG2a (top) and IgG2c (bottom) deposition in glomeruli of kidneys from a homozygous 564Igi K− control, a homozygous 564Igi, a homozygous 564Igi mouse, and a 564Igi mixed chimera. Glomerular vessels defined by CD31 (red), nuclei counterstained with Hoechst (blue), Ab deposits in green.
Article Snippet: Commercial antibodies and staining reagents, from BioLegend: GL7-PacBlue, GL7-PE, GL7-A647, B220-PerCP-Cy5.5, B220-APC-Cy7, IgMb-FITC, IgMa-PE, CD45.2-FITC, CD45.2-APC, CD45.1-FITC, CD45.1-PE, IgD-PB, CD21/35 (7E9)-PE, CD138-PE, CD38-PE-Cy7, CD31-A647, CD157-PE, Streptavidin-PE/Cy7; from eBioscience: CD95 (APO-1/Fas)-PE (clone 15A7) and viability dye Fixable live/dead stain Efluor780; from ThermoFisher Scientific: Rabbit-anti-Goat-A488, Hoechst 33342, DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride), Phalloidin-A568; from Southern Biotech: AP-Goat-anti-Mouse IgG, AP-Goat-anti-Mouse IgG2a, AP-Goat-anti-Mouse
Techniques: Immunofluorescence, Control